Deletion of the Scl 19 enhancer increases the blood stem cell Biology Diagrams Fucci (CA) yields abundant expression of mCherry throughout G1 phase with an abrupt decrease in the red fluorescence at the end of G1 phase. Fucci (CA) can be used to reliably detect a short G1 phase and distinguish S and G2 phases, which previously was difficult to achieve. (CA) Figure1: Visualization of Cell-Cycle Progression by Fucci (SA
Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator)is a set of fluorescent probes which enable the visualization of cell cycle progression in living cells. Fucci takes advantage of the fact that the replication licensing factors Cdt1 and Geminin are only present during specific phases of the cell cycle. A fusion protein of a fragment
Visualizing Spatiotemporal Dynamics of Multicellular Cell Biology Diagrams
The expression levels of two closely linked proteins in the eukaryotic DNA replication network came in handy for Miyawaki's team when visualizing the cell cycle. Chromatin licensing and DNA replication factor 1 (Cdt1) "licenses" DNA to replicate during the cell cycle. Geminin inhibits Cdt1, preventing it from re-replicating DNA. By visualizing the cell cycle, Fucci is a powerful tool to investigate any process that has to do with cell growth and differentiation, such as the development and regeneration of organs as well as carcinogenesis. Cells with pure green fluorescence were either in the S or G2 phase and were distinguished by immunostaining of the S phase. The
The widely used fluorescence ubiquitination cell cycle indicator (FUCCI) system, based on the expression of the cell cycle oscillators Cdt1 and geminin tagged with different fluorescent proteins Abstract. Proper cell-cycle progression is essential for the self-renewal and differentiation of human pluripotent stem cells (hPSCs). The fluorescent ubiquitination-based cell-cycle indicator (FUCCI) has allowed the dual-color visualization of the G 1 and S/G 2 /M phases in various dynamic models, but its application in hPSCs is not widely reported. In addition, lineage-specific FUCCI A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and
color cell cycle indicator for fluorescence ... Biology Diagrams
The fluorescence ubiquitination cell-cycle indicator (FUCCI) was developed in 2008 by Miyawaki et al., which color-codes the phases of the cell cycle in real-time. Therefore, visualizing cell cycle dynamics within tumors with FUCCI can provide a guide for many strategies to improve cell-cycle targeting therapy for solid cancers. Keywords:
